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将单壁碳纳米管-壳聚糖的复合材料(SWCNTs-CS)滴涂在玻碳电极后,采用恒电位沉积的方法在SWCNTs-CS上生成纳米铂,形成Pt NPs-SWCNTsCS复合物后用以固定癌胚抗原抗体(anti-CEA)制成免疫传感界面,在癌胚抗原(CEA)的存在下,在电极表面形成抗原-抗体免疫复合物,以[Fe(CN)6]3-/4-作为混合探针电解质,构建一种简单灵敏无标记的阻抗型免疫分析方法。随着固定CEA量增加,传感器上传递电子的能力下降导致阻抗值增大,且CEA浓度在0.010~12 ng/m L范围时,与阻抗值呈现出良好的线性关系,线性方程为△Retohm=(831.3±17.8)cCEA(ng/m L)+(885.8±95.1)(R2=0.997),检测限为4.2 pg/m L(S/N=3)。该免疫传感器具有良好的稳定性和选择性,回收率在95.0%~108.6%,可初步用于临床对CEA的检测。
After single-walled carbon nanotube-chitosan composites (SWCNTs-CS) were applied onto glassy carbon electrode, platinum nanoparticles were formed on SWCNTs-CS by potentiostatic deposition and Pt NPs-SWCNTsCS complexes were formed Immune sensory interface was made by immobilized anti-CEA, and antigen-antibody immune complex was formed on the surface of electrode in the presence of carcinoembryonic antigen (CEA). [Fe (CN) 6] 3- / 4- as a hybrid probe electrolyte, to construct a simple, sensitive and unlabeled impedance immunoassay. With the increase of immobilized CEA, the decrease of the ability of the sensor to transmit electrons led to the increase of the impedance value. When the concentration of CEA was in the range of 0.010-12 ng / m L, the impedance value showed a good linear relationship with the linear equation △ Retohm = (831.3 ± 17.8) cCEA (ng / m L) + (885.8 ± 95.1) (R2 = 0.997). The detection limit was 4.2 pg / m L (S / N = 3). The immunosensor has good stability and selectivity, and the recovery rate is between 95.0% and 108.6%. It can be used clinically for the detection of CEA.