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抽穗期(heading data,HD)和株高(plant height,PH)是水稻(Oryza sativa L.)重要的农艺性状。本研究利用金23B(Jin23B)和CR071构建了BC3F1群体及其衍生的BC3F2群体,建立的遗传连锁图谱包含140对SSR标记和5对InDel标记,较好的覆盖了水稻的12条染色体。两年共定位到了12个抽穗期相关QTLs(quantitative trait loci),8个株高相关的QTLs。其中抽穗期和株高最大效应QTL均由CR071提供,且都定位于第7染色体同一位置:抽穗期QTL qHD7 2011年LOD为33.26,解释的表型贡献率为38.2%,加性效应为10.40;2012年LOD为31.66,可以解释的表型贡献率为30.3%,加性效应为12.28;株高QTL qPH7同2011年LOD为48.72,解释的表型贡献率为44.2%,加性效应为19.97;2012年LOD为55.78,可以解释的表型贡献率为56.7%,加性效应为17.73。qHD7和qPH7都位于RM501-RM542之间,Ghd7也位于这一区间,该QTL可能就是Ghd7。抽穗期QTL qHD1-2和株高QTL qPH1-2同样都位于RM572-RM562之间,可以延迟抽穗期5 d左右和增加株高5 cm左右,qHD1-2两年内的LOD值分别为:7.58和7.03,可以解释的表型变异率分别为:6.84%和5.21%;qPH1-2两年内的LOD值分别为:4.21和4.77,可以解释的表型变异率为3.20%和3.64%,这一位点没有相关QTL定位或基因克隆的报道,位于这一区间的这两个性状也可能是由同一基因控制的。本研究通过发掘新的抽穗期和株高QTL,来增加基因的资源的多样性,为育种家利用分子标记辅助选择培育新品种提供更多的选择。
Heading date (HD) and plant height (PH) are important agronomic traits in rice (Oryza sativa L.). In this study, the BC3F1 population and its derived BC3F2 population were constructed using Jin23B (Jin23B) and CR071. The established genetic linkage map contained 140 SSR markers and 5 pairs of InDel markers, covering 12 rice chromosomes. In the past two years, 12 QTLs (heading trait loci) and eight QTLs related to plant height were mapped. The QTLs for both heading date and plant height were all provided by CR071 and all located at the same position on chromosome 7: the QTL qHD7 at heading stage was 33.26 in 2011, the phenotypic contribution rate was 38.2% and the additive effect was 10.40; In 2012, the LOD was 31.66, the phenotypic contribution rate was 30.3% and the additive effect was 12.28. The QTL for plant height QPH7 was 48.72 with the LOD in 2011, explaining the phenotypic contribution rate of 44.2% and the additive effect of 19.97. The LOD was 55.78 in 2012, with a phenotypic contribution rate of 56.7% and an additive effect of 17.73. Both qHD7 and qPH7 are located between RM501 and RM542, and Ghd7 is also located in this interval. This QTL may be Ghd7. QTL qHD1-2 and QTL qPH1-2 at heading stage were also located between RM572 and RM562, which could delay heading about 5 days and increase plant height about 5 cm. LOD values of qHD1-2 in two years were 7.58 and 7.03, respectively. The phenotypic variation rates were 6.84% and 5.21% respectively. The LOD values of qPH1-2 in two years were 4.21 and 4.77, respectively. The phenotypic variation rates were 3.20% and 3.64% There are no reports of related QTL mapping or gene cloning, and the two traits in this interval may also be controlled by the same gene. In this study, by exploring new QTLs for heading date and plant height to increase the diversity of genetic resources, breeders can use molecular marker-assisted selection to cultivate new varieties to provide more choices.