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目的 探讨nm2 3 H1基因转染对人高转移大细胞肺癌细胞株L9981中 β catenin和磷酸化β catenin表达水平的影响 ,为阐明nm2 3 H1基因逆转肺癌转移的分子机制提供实验依据。方法 以原代L9981、转染nm 2 3 H1基因的L9981 nm2 3 H1和转染空载体的L9981 pLXSN三株肺癌细胞株为研究对象 ,应用WesternBlot检测比较各细胞株胞浆、胞核中 β catenin和磷酸化 β catenin表达水平的变化 ,以确定nm 2 3 H1基因转染是否能调控人高转移大细胞肺癌细胞株L9981中 β catenin和磷酸化 β catenin表达。 结果 ①β catenin在L9981 nm2 3 H1细胞株胞浆中表达量 (IOD) (3 64 9± 118)显著高于L9981(14 0 1± 3 1)和L9981 pLXSN(13 5 0± 5 5 )细胞株 (P <0 .0 0 1) ;②β catenin在L9981 nm 2 3 H1细胞株胞核中表达量 (2 945± 68)与L9981(2 60 4± 2 3 )和L9981 pLXSN(2 65 2± 5 3 )细胞株比较均无显著性差异 (P >0 .0 5 ) ;③磷酸化β catenin在L9981 nm 2 3 H1细胞株胞浆中表达量 (3 12 3± 10 2 )显著低于L9981(4 3 62± 13 1)和L9981 pLXSN (4 5 0 0±117)细胞株 (P <0 .0 0 1) ;④磷酸化 β catenin在L9981 nm2 3 H1细胞株胞核中表达量 (5 13 6± 112 )显著高于L9981(2 666± 116)和L9981 pLXSN(2 661±
Objective To investigate the effect of nm23 H1 gene transfection on the expression of β catenin and phospho-β catenin in human high metastatic large cell lung cancer cell line L9981 and provide the experimental evidence for elucidating the molecular mechanism of nm23 H1 gene reversing lung cancer metastasis. Methods Primary L9981, L9981 nm2 3 H1 transfected with nm 2 3 H1 gene and L9981 pLXSN lung cancer cell line transfected with empty vector were used as research objects. Western blot was used to detect the expression of β catenin in cytoplasm and nucleus of each cell line And phosphorylated β catenin expression levels to determine whether nm 2 3 H1 gene transfection can regulate human high metastatic large cell lung cancer cell line L9981 β catenin and phosphorylated β catenin expression. Results ① The expression of β catenin in cytoplasm of L9981 nm23 H1 cell line (3 64 9 ± 118) was significantly higher than that of L9981 (14 0 1 ± 3 1) and L9981 pLXSN (105 0 ± 5 5) cell lines (P <0.01). ② The expression of β catenin in the nucleus of L9981 nm 2 3 H1 cells (2 945 ± 68) was significantly higher than that of L9981 (2 60 4 ± 2 3) and L9981 pLXSN (3) The cell lines showed no significant difference (P> 0.05); ③ The expression of phosphorylated catenin in cytoplasm of L9981 nm 2 3 H1 cell line was significantly lower than that of L9981 (3 12 3 ± 10 2) 4 3 62 ± 13 1) and L9981 pLXSN (45 00 ± 117) cell lines (P 0 01). ④ The expression of phosphorylated catenin in the nucleus of L9981 nm2 3 H1 cell line (5 13 6 ± 112) was significantly higher than that of L9981 (2 666 ± 116) and L9981 pLXSN (2 661 ±