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我们用枯草杆菌H_(17)及M_(45)菌株芽胞DNA重组试验(Rec—Assay)的划线法和混合法。分别在冷孵(4～5℃24小时)和不冷孵条件下。测定了六个直接突变物和/或致癌物(EMS,灭菌丹,丝裂霉素,HgCl_2,K_2Cr_2O_7K_2Cr_2O_4)和两个间接致突变物和/或致癌物(吖叮橙,AFB_1)的重组效应。并比较了它们对所用测试菌株的敏感性。其结果表明,六个直接致突变物和/或致癌物对测试菌株用划线法冷孵比混合法冷孵有较高的重组效应;另两个间接致突变物和/或致癌物经S9活化后用混合法立即置37℃20小时比划线法有更高的敏感性,
We used the Bacillus subtilis H_ (17) and M_ (45) strains spore recombination assay (Rec-Assay) scribing and mixing method. Respectively, in the cold incubation (4 ~ 5 ℃ 24 hours) and non-incubation conditions. The recombinant effects of six direct mutants and / or carcinogens (EMS, bacteriocin, mitomycin, HgCl_2, K_2Cr_2O_7K_2Cr_2O_4) and two indirect mutagens and / or carcinogens (acridine orange, AFB_1) . And compared their sensitivity to the tested strains used. The results showed that the six direct mutagens and / or carcinogens had a higher recombination effect on the tested strains by cold hatching than the mixed incubation method; the other two indirect mutagens and / or carcinogens were S9 Activated immediately after mixing at 37 ℃ for 20 hours than the underline method has a higher sensitivity,