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我们用以下方法研究了胺菊酯(合成拟除虫菊酯)的遗传毒性:①非程序性DNA合成(UDS)检测胺菊酯对FL细胞DNA的损伤作用;②平板掺入试验及波动试验检测由胺菊酯诱发的鼠伤寒沙门氏菌的回复突变。结果:①胺菊酯浓度5×10~(-2)～5×10~0μg/ml,不论检测系统中是否加入S9混合物均诱发FL细胞的UDS,并呈剂量反应关系;②在平板试验中,如检测系统中不加S9混合物,胺菊酯(5、50及500μg/皿)不诱发TA100、TA98及TA97的回复突变;加入S9混合物,胺菊酯(500μg/皿)使TA100及TA97的回度菌落数增加至对照的两倍左右;③在波动试验中,检测系统不论是否加进S9混合物,胺菊酯(50及500μg/ml)诱发的TA97的阳性管数均显著高于对照管。上述结果证实胺菊酯对哺乳类细胞的DNA有损伤作用并对鼠伤寒沙门氏菌有诱变性,我们认为,如将UDS检测与Ames试验结合,必要时辅以波动试验,可提高对致突变性和致癌性化合物在初筛中的检测价值。
We studied the genotoxicity of tetramethrin (synthetic pyrethroid) by the following methods: (1) non-programmed DNA synthesis (UDS) was used to detect the DNA damage of tetramethrin against FL cells; (2) Reversible mutations in tetramethrin induced Salmonella typhimurium. Results: ① The concentration of tetramethrin was 5 × 10 -2 ~ 5 × 10 ~ 0 μg / ml, regardless of whether S9 mixture was added into the test system, the UDS of FL cells was induced and the dose-response relationship was observed. ② In the plate test For example, tetramethrin (5, 50 and 500 μg / dish) did not induce the reverse mutation of TA100, TA98 and TA97 in the detection system. S9 mixture and tetramethrin (500 μg / dish) The number of returned colonies increased to about twice that of the control. (3) In the fluctuation test, the number of TA97 positive cells induced by tetramethrin (50 and 500 μg / ml) was significantly higher than that of the control without or with S9 mixture . The above results confirm that tetramethrin has a damaging effect on DNA of mammalian cells and is mutagenic to S. typhimurium. In our opinion, if the UDS test is combined with the Ames test, if necessary, the fluctuation test may be supplemented to increase the mutagenicity And carcinogenic compounds in the initial screening of the value.