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目的:优选秦岭龙胆中齐墩果酸的乙醇回流提取工艺。方法:采用HPLC测定齐墩果酸含量,流动相乙腈-甲醇-水-磷酸-三乙胺(25∶15∶10∶0.01∶0.02),检测波长210 nm。在单因素试验基础上,根据Box-Benhnken中心组合设计原理,利用三因素三水平响应面分析法考察料液比、提取次数、提取时间对齐墩果酸提取量的影响。结果:最佳提取工艺为料液比1∶12,提取数2次,提取时间76 min;齐墩果酸提取量0.583 mg·g-1,与预测值0.605 mg·g-1的偏差仅3.64%。结论:采用响应面法优选的秦岭龙胆回流提取工艺稳定可行,为秦岭龙胆的资源开发及临床推广提供参考。
Objective: To optimize the extraction of oleanolic acid from Gentiana gentiana in Qinling. Methods: The content of oleanolic acid was determined by HPLC. The mobile phase was acetonitrile - methanol - water - phosphoric acid - triethylamine (25:15:10:0.01:0.02). The detection wavelength was 210 nm. Based on the single-factor experiment, according to the design principle of Box-Benhnken central composite design, the effect of the ratio of oleanolic acid to the extraction ratio of material-liquid ratio, extraction times and extraction time was investigated by three-factor three-level response surface analysis. Results: The optimum extraction process was the ratio of solid to liquid 1:12, extraction 2 times, extraction time 76 min, the yield of oleanolic acid 0.583 mg · g-1, and the deviation from the predicted value of 0.605 mg · g-1 was only 3.64 %. Conclusion: The extraction process of gentian from Qinling Mountain by response surface methodology is stable and feasible, which provides a reference for resource development and clinical promotion of gentian.