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用ＥＬＩＳＡ方法检测了１６５例不同年龄组正常人血清中痘苗抗体水平，结果１２岁以下儿童痘苗病毒ＩｇＧ抗体全部阴性，１３岁以上各年龄组痘苗ＩｇＧ抗体阳性率在５３．８～８０．０％左右，阳性抗体效价分布在１∶２００～１∶１６００之间，几何平均滴度为１∶２６９～１∶２９５之间。虽然３１岁～４０岁年龄组痘苗ＩｇＧ抗体阳性率略有升高，但各年龄组之间痘苗抗体几何平均滴度无显著差异（Ｐ＞０．０５）。用间接免疫荧光方法检测，结果１～１２岁年龄组痘苗病毒ＩｇＧ抗体全部阴性，与ＥＬＩＳＡ方法检测结果一致，１３岁以上１００例血清中痘苗抗体阳性数２０例，阳性率２０％，但不同年龄组之间阳性率有所变化，但无明显差异。
The ELISA method was used to detect the level of vaccinia antibody in serum of 165 normal persons of different age groups. The results showed that all the vaccinia virus IgG antibodies were negative in children under 12 years old. The positive rate of vaccinia IgG antibody in all age groups over 13 years old was 53.8-80.0% , The titer of the positive antibody is distributed between 1: 200 and 1: 1600, and the geometric mean titer is between 1: 269 and 1: 295. Although the positive rate of IgG antibodies to vaccinia in 31- 40 age group increased slightly, geometric mean titer of vaccinia antibody was not significantly different among all age groups (P> 0.05). Indirect immunofluorescence assay, the results of 1 to 12-year-old vaccinia virus IgG antibodies all negative, and ELISA test results, 100 cases of serum antibodies over the age of 13 in 20 cases of positive, the positive rate of 20%, but different ages The positive rate between the groups changed, but no significant difference.