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人类免疫缺陷病毒Ⅰ型（ＨＩＶ－１）核心抗体的检测对于ＨＩＶ－１感染者的筛选及确证分析，感染病人的预后分析都具有重要的意义。为此需要获得大量重组核心蛋白。本文利用新型原核表达载体ｐＤＯＧ，在大肠杆菌中高效表达了人类免疫缺陷病毒Ⅰ型（ＨＩＶ－１）ｇａｇ基因片
The detection of human immunodeficiency virus type 1 (HIV-1) core antibody is of great significance for the screening and confirmatory analysis of HIV-1 infected patients and for the prognosis analysis of infected patients. To do this requires a large amount of recombinant core protein. In this study, a new type of prokaryotic expression vector pDOG was constructed and used to express human immunodeficiency virus type 1 (HIV-1) gag gene in Escherichia coli