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采用兔抗人ＣｙｓｔａｔｉｎＣ抗体建立了酶联免疫吸附测定（ＥＬＩＳＡ）技术，对急性心肌梗塞病人急性发作期、恢复期及正常对照进行了测定。发现心梗急性发作期和恢复期血清ｃｙｓｔａｔｉｎＣ含量无明显变化，但均明显低于对照（Ｐ＜０．０ｌ）。用Ｌｏｇｉｓｔｉｃ回归模型校正年龄因素的影响后，上述差别仍然显著（Ｐ＝０．０４１）。提示血清ｃｙｓｔａｔｉｎＣ浓度变化在一定程度上可作为急性心肌梗塞诊断的参考指标。此检测方法特异性强、灵敏度高、重复性好。
Rabbit anti-human CystatinC antibody was used to establish the enzyme-linked immunosorbent assay (ELISA) for the determination of acute exacerbation, convalescence and normal control in patients with acute myocardial infarction. It was found that there was no significant change of serum cystatin C in acute attack and convalescent phase of myocardial infarction, but both were significantly lower than that of control (P <0.01). Logistic regression model adjusted for age factors, the difference is still significant (P = 0.041). Tip serum cystatinC concentration changes to a certain extent, can be used as a reference indicator for the diagnosis of acute myocardial infarction. This test method is specific, sensitive, reproducible.