论文部分内容阅读
本实验共用成年雄性大鼠40只,分为溃疡组、盐水对照组和空白对照组。溃疡组在无菌条件下,将0.01ml冰醋酸注入胃粘膜下层,造成实验性胃溃疡,盐水组模拟手术,注入生理盐水0.01ml;空白组为未经处理的正常大鼠。三组动物于相同条件下饲养。术后6、10、14、21天分别取材,每批动物均包括溃疡组5只、盐水组3只和空白组2只。用免疫细胞化学PAP法,分别显示胰岛A、B细胞,
In this experiment, 40 adult male rats were divided into ulcer group, saline control group and blank control group. Under aseptic conditions, the ulcer group injected 0.01 ml of glacial acetic acid into the submucosa of the stomach, resulting in experimental gastric ulcers. The saline group received a simulated operation and received 0.01 ml of physiological saline. The blank group was untreated normal rats. Three groups of animals were reared under the same conditions. 6 days, 10 days, 14 days and 21 days after operation, each group of animals included 5 ulcers, 3 saline and 2 blanks. Immunocytochemical PAP method was used to show islet A and B cells respectively.