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淀粉颗粒结合蛋白包含了多种淀粉生物合成的关键酶,对作物淀粉品质有重要影响。本研究利用1D-SDS-PAGE,分离了74份四川、西藏及云南毗邻地区小麦的淀粉颗粒结合蛋白,对突变材料进行了分子标记检测,对总淀粉和直链淀粉含量差异进行了比较。发现供试材料中,在分子量57～130 kDa区域共有9种不同的蛋白条带。其中,2个条带可能为新的淀粉颗粒结合蛋白;存在12份Wx-B1缺失的自然突变体和3份稀有的SGP-B1缺失突变体;筛选到直链淀粉含量超过30%的材料2份,直链淀粉含量为15%左右的材料10份。这些材料为小麦淀粉品质改良及淀粉生化合成机理研究提供了基础。
Starch granule-binding protein contains many key enzymes of starch biosynthesis, and has an important impact on crop starch quality. In this study, we isolated 74 starch binding proteins from wheat in adjacent areas of Sichuan, Tibet and Yunnan by 1D-SDS-PAGE. The mutants were detected by molecular markers, and the differences of total starch and amylose content were compared. A total of 9 different protein bands were found in the region of 57 ~ 130 kDa molecular weight. Among them, the two bands may be new starch granule-binding proteins; there are 12 natural mutants with Wx-B1 deletion and 3 rare SGP-B1 deletion mutants; screening material 2 with amylose content more than 30% Parts, amylose content of about 15% of the material 10 copies. These materials provide the basis for the research of wheat starch quality improvement and starch biochemical synthesis mechanism.