论文部分内容阅读
采用固相pH梯度/SDS-PAGE双向凝胶电泳对经杀雄剂SQ-1处理和未处理的小麦(Triticum aestivum)成熟期花粉总蛋白质进行了分离,银染显色,获得了分辨率和重复性较好的双向电泳图谱.通过PDQuest 2DE图像软件的分析,在等电点4～7之间可识别350个以上较为清晰的蛋白质点,其中差异表达明显的蛋白质点数为21个.将11个差异点采用基质辅助激光解析电离飞行时间质谱进行了肽质量指纹图谱分析,采用Mascot软件在Swiss-prot数据库查询,鉴定出了7个蛋白质,它们分别是液泡转化酶、动力蛋白轻链TCTEX-1、锰超氧化物歧化酶、果糖-1,6-二磷酸醛缩酶、抗坏血酸过氧化物酶、凝集素蛋白激酶和一种未知功能的蛋白.对已知蛋白的功能进行分析,推测杀雄剂SQ-1诱导小麦雄性不育可能与能量代谢失衡、淀粉合成受抑制、活性氧积累、细胞凋亡以及花器官发育调节基因作用失控等有关.
Solid phase pH gradient / SDS-PAGE two-dimensional gel electrophoresis was used to separate the mature pollen proteins from the antisera of SQ-1 and untreated wheat (Triticum aestivum) Repeatability of the two-dimensional electrophoresis.PDQuest 2DE image software analysis, in the isoelectric point of 4 to 7 can be identified more than 350 clear protein spots, of which the number of differentially expressed proteins is 21. Will be 11 The differences were analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MS-MS / MS). The peptides were characterized by Mascot software in Swiss-prot database. Seven proteins were identified as vacuolar invertase, dynein light chain TCTEX- 1, manganese superoxide dismutase, fructose-1,6-bisphosphate aldolase, ascorbate peroxidase, lectin protein kinase and an unknown function protein.Analysis of the function of the known protein speculated that killing Male sterility induced by SQ-1 may be related to unbalance of energy metabolism, inhibition of starch synthesis, accumulation of reactive oxygen species, apoptosis and uncontrolled gene function of flower organ development.