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用草酸毒素作筛选剂处理油菜愈伤组织,获得耐毒素的突变体,分化培养后得到小量再生植株。经用毒素处理离体叶片,油菜菌核病菌菌丝体接种离体叶片和田间植株及田间病圃测定几种方法鉴定显示,所获再生植株与原品系(2242)相比较,均不同程度提高了对菌核病的抗性,其F1代材料保持了抗性,F2代材料抗性有一定的分化,但大部分的仍保持了抗性,其中有S11、S21、S23、S24、S25、S26几个材料具有较明显的抗性,可用作抗源和进一步选育抗病良种。
Oxalic acid toxin screening agent for processing rape callus, to be resistant to mutants, differentiation and culture to get a small amount of regenerated plants. Isolation of detached leaves by using toxins, inoculation of isolated rape mycelia of S. sclerotiorum and in-field nurseries and in-field nurseries were evaluated by several methods. The results showed that the regenerated plants were all increased to varying degrees compared with the original strain (2242) Resistant to Sclerotinia sclerotiorum, the material of F1 was kept resistant, the resistance of F2 was somewhat differentiated, but most of them remained resistant, including S11, S21, S23, S24, S25, S26 several materials have more obvious resistance, can be used as a source of resistance and further breeding resistant varieties.