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目的分析四川省遂宁市自然人群中乙型肝炎(乙肝)病毒(Hepatitis B Virus,HBV)主要基因型S基因主要亲水区[Major Hydrophilic Region,MHR;氨基酸(Amino Acid,aa)99-169]及人类白细胞抗原I型(The Putative Human Leukocyte Antigen Class I-Restricted,HLA-I)细胞毒性T淋巴细胞(Cytotoxic T Lymphocyte,CTL)表位aa位点置换特点。方法遂宁市乙肝血清流行病学调查中乙肝病毒表面抗原阳性人群,扩增HBV S基因片段并测序得到的完整S基因片段序列,进行生物信息学比对分析。结果在遂宁市共得到108份序列,其中B基因型40份,C基因型68份,在S基因MHR发生aa置换位点为Y100C、Q101H、L110F、T126A/S、T131I、S136F、T143M、G145R、V168A,在HLA-I型CTL表位aa置换位点为V47A、P46L、T47K、L88P、I92T、L98V、Y100C、Q101H、V177A、V194A,位点置换在年龄分布和基因型分布的差异无统计学意义。结论遂宁市HBV S基因MHR及HLA-I型CTL表位aa位点置换与基因型分布无关,未发现接种乙肝疫苗与人群中HBV S区aa位点置换相关。
Objective To analyze the major Hydrophilic Region (MHR) amino acid (aa) 99-169 of S gene of major genotypes of Hepatitis B Virus (HBV) in natural population of Suining City, Sichuan Province. And the Putative Human Leukocyte Antigen Class I-Restricted (HLA-I) Cytotoxic T Lymphocyte (CTL) epitope aa site. Methods Suining hepatitis B seropositive survey hepatitis B virus surface antigen positive population, amplified HBV S gene fragment and sequenced the complete S gene fragment sequence, bioinformatics analysis. Results A total of 108 sequences were obtained in Suining city, including 40 genotypes of B and 68 genotypes of C genotypes. The aa substitution sites of MHR gene S were Y100C, Q101H, L110F, T126A / S, T131I, S136F, T143M, G145R , V168A, there was no statistical difference in the age distribution and genotype distribution between the substitution sites of HLA-I CTL epitope aa V47A, P46L, T47K, L88P, I92T, L98V, Y100C, Q101H, V177A, V194A Significance of learning. Conclusion Suining City, the S gene of MHR and HLA-I type CTL epitope aa site replacement has nothing to do with the genotype distribution, did not find vaccinated hepatitis B vaccine and the crowd in the HBV S aa site replacement related.