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目的预测内皮细胞特异分子-2(endothelial cell-specific molecule-2,ECSM2)胞外区B细胞表位。方法以ECSM2胞外区氨基酸序列为基础,应用公共的生物信息学工具,分析ECSM2的序列特异性、二级结构,以及极性、亲水性、柔韧性、表面可及性等参数,然后借助专业的B细胞表位预测工具预测潜在的B细胞表位,最后对获得的数据综合分析,评判ECSM2胞外区最有可能的B细胞表位。结果综合分析多种生物信息学工具获得的数据表明,ECSM2胞外区69-93位氨基酸区段包含了4个相互重叠的潜在表位,具有极大的B细胞表位可能性。结论应用生物信息学预测出的ECSM2胞外区B细胞表位,为直接利用该表位从抗体库或杂交瘤细胞库中筛选出血管内皮细胞靶向的特异抗体提供了理论依据。
Objective To predict the extracellular B cell epitopes of endothelial cell-specific molecule-2 (ECSM2). Methods Based on the amino acid sequence of extracellular domain of ECSM2, the sequence specificity, secondary structure, polarity, hydrophilicity, flexibility, surface accessibility and other parameters of ECSM2 were analyzed by using public bioinformatics tools. A professional B cell epitope prediction tool predicts potential B cell epitopes. Finally, a comprehensive analysis of the data obtained is performed to determine the most likely B cell epitopes in the ECSM2 extracellular domain. Results A comprehensive analysis of the data obtained from various bioinformatics tools showed that the amino acid segment 69-93 in the ECSM2 extracellular domain contains four potential epitopes that overlap each other and have a great potential for B cell epitopes. Conclusion The B cell epitope of extracellular region of ECSM2 predicted by bioinformatics provides a theoretical basis for the direct screening of specific antibodies targeted by vascular endothelial cells from the library of antibody or hybridoma using this epitope.