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目的探讨星状神经节阻滞对肾缺血-再灌注大鼠模型肾组织损伤的保护作用及作用机制。方法健康成年SD大鼠30只,随机分为3组,每组10只:假手术组(Sham组)、缺血-再灌注组(IR组)、缺血-再灌注+颈交感神经干离断组(IR+TCST组)。手术后48 h测各组大鼠血肌酐(S_(cr))、尿素氮(BUN)含量及尿量的变化;取大鼠肾组织,观察形态结构变化;westeron blot检测肾组织中Bcl-2和Bax蛋白的表达水平。结果 IR组大鼠血浆中S_(cr)和BUN均明显增高,尿量减少,可见广泛的肾小管上皮细胞发生凋亡,Bcl-2蛋白含量减少而Bax蛋白含量升高,与Sham组相比,差异有统计学意义(P<0.05);IR+TCST组大鼠血浆S_(cr)和BUN均明显降低,尿量增加,肾组织中肾小管上皮细胞凋亡数量减少,Bcl-2蛋白含量升高而Bax蛋白含量减少,与IR组相比,差异有统计学意义(P<0.05)。结论星状神经节阻滞对大鼠肾缺血-再灌注损伤具有保护作用,其机制是通过增加肾组织中Bcl-2蛋白的表达量,降低Bax蛋白的表达水平来抑制肾小管上皮细胞凋亡。
Objective To investigate the protective effect of stellate ganglion block on renal tissue injury induced by renal ischemia-reperfusion in rats and its mechanism. Methods Thirty healthy adult Sprague-Dawley rats were randomly divided into 3 groups with 10 rats in each group: Sham group, ischemia-reperfusion group (IR group), ischemia-reperfusion + Broken group (IR + TCST group). The levels of serum creatinine (S_ (cr)), blood urea nitrogen (BUN) and urine volume were measured at 48 h after operation in rats. The renal tissues of rats were sacrificed and the morphological changes were observed. The expressions of Bcl-2 And Bax protein expression levels. Results The levels of S_ (cr) and BUN in plasma of rats in IR group were significantly higher than those in Sham group (P <0.05), and the urine output was decreased. The apoptosis of tubular epithelial cells was observed in B group and decreased in Bcl-2 protein and Bax protein , The difference was statistically significant (P <0.05). The levels of S_ (cr) and BUN in plasma of rats in IR + TCST group were significantly decreased, the urine output increased, the number of renal tubular epithelial cell apoptosis and the content of Bcl- Compared with the IR group, the difference was statistically significant (P <0.05). Conclusions Stellate ganglion block has a protective effect on renal ischemia-reperfusion injury in rats by inhibiting the expression of Bcl-2 protein and decreasing the expression of Bax protein in renal tissue Death.