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目的:建立反相高效液相色谱紫外法测定人血浆中甲哌卡因浓度。方法:采用Diamonsil ODS C_(18)分析柱(150 mm×4.6 mm,5μm),柱温为室温,流动相为甲醇-乙腈-0.03 mol·L~(-1)KH_2PO_4缓冲液(H_3PO_4调pH为5.3)(50:50:250),流速:1.0ml·min~(-1);0.5 ml血浆样品经固相萃取方法处理后,洗脱液在50℃条件下空气吹干,残渣用流动相溶解进样,紫外检测器检测,检测波长为210 nm。结果:标准曲线在20～4 000 ng·ml~(-1)范围内线性良好,最低定量限为20 ng·ml~(-1),甲哌卡因及内标的保留时间分别为7.5 min和19.4 min,日内和日间RSD均<14.0%,方法提取回收率分别在103.5%～109.2%。结论:本法具有简便、灵敏、准确等优点,成功用于经硬膜外给予200 mg盐酸甲哌卡因患者血浆液中甲哌卡因浓度测定及药动学研究。
Objective: To establish a reversed-phase high performance liquid chromatographic method for the determination of mepivacaine in human plasma. METHODS: Diamonsil ODS C 18 column (150 mm × 4.6 mm, 5 μm) was used. The column temperature was at room temperature and the mobile phase was methanol-acetonitrile-0.03 mol·L -1 KH 2 PO 4 buffer (H_3PO_4 adjusted to pH = (50:50:50), the flow rate was 1.0ml · min -1. After 0.5 ml plasma sample was treated by solid-phase extraction, the eluate was air-dried at 50 ° C. The residue was washed with mobile phase Dissolved injection, UV detector detection, detection wavelength of 210 nm. Results: The calibration curve was linear in the range of 20-4000 ng · ml -1 with the lowest limit of quantitation of 20 ng · ml -1. The retention times of mepivacaine and internal standard were 7.5 min and 19.4 min, RSD both in day and day were <14.0%, and the recoveries were in the range of 103.5% -109.2%. Conclusion: The method is simple, sensitive and accurate. It is successfully used in the determination of mepivacaine in plasma of 200 mg mepivacaine hydrochloride by epidural administration and its pharmacokinetics.