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白肉灵芝(Ganoderma leucocontextum)栽培中,菌种的真实性问题一直是制约其栽培成功率的主要因素之一,为了快速准确地鉴定白肉灵芝,本研究对白肉灵芝、灵芝(赤芝,G.lingzhi)、紫芝(G.sinense)、漆光灵芝(亮盖灵芝,G.lucidum)、四川灵芝(G.sichuanense)、日本灵芝(G.japonicum)、树舌(G.applanatum)、有柄树舌(G.gibbosum)、弯柄灵芝(G.flexipes)、无柄灵芝(G.resinaceum)和紫铜灵芝(G.cupreum)等共17个灵芝属样品进行了ITS序列测定分析比对,从而发现白肉灵芝ITS序列在120~140 bp以及450~470 bp处有多个特异碱基位点,基于这些特异碱基位点设计白肉灵芝的特异引物对BRLZ-F和BRLZ-R,结果表明,该特异引物对在58℃退火温度下特异性好,仅能扩增出白肉灵芝相应的长348 bp的特异片段。因此该特异引物对及方法可用于白肉灵芝的快速准确鉴定。
Ganoderma lucidum (Ganoderma leucocontextum) cultivation, the authenticity of the strain has been a major factor restricting the success rate of its cultivation, in order to quickly and accurately identify white meat Ganoderma lucidum, white fungus Ganoderma lucidum (Ganoderma lucidum, G. lingzhi) G. sinense, G. lucidum, G. sichuanense, G. japonicum, G. applanatum, G.gbbosum, G.flexipes, G.resinaceum and G.cupreum were sequenced and their ITS sequences were compared and analyzed. As a result, it was found that Ganoderma lucidum ITS sequences have multiple specific base sites at 120-140 bp and 450-470 bp. Based on these specific base sites, specific primers of BRLZ-F and BRLZ-R were designed, and the results showed that the specific primers Specificity was good at the annealing temperature of 58 ℃, and only the corresponding 348 bp specific fragment of white ganoderma lucidum could be amplified. Therefore, the specific primer pair and the method can be used for rapid and accurate identification of white ganoderma lucidum.