【摘 要】
：
Tcmporins是从蛙属中得到的一类羧基端酰胺化的疏水性抗菌肽,具有抗细菌、霉菌、酵母菌、原虫及病毒活性.为丫研究牛蛙皮肤抗菌肽的多样性及其结构特点,根据GenBank数据库中
【机 构】
：
吉林大学畜牧兽医学院,中国科学院动物研究所,中国医学科学院实验动物研究所
论文部分内容阅读
Tcmporins是从蛙属中得到的一类羧基端酰胺化的疏水性抗菌肽,具有抗细菌、霉菌、酵母菌、原虫及病毒活性.为丫研究牛蛙皮肤抗菌肽的多样性及其结构特点,根据GenBank数据库中蛙属抗菌肽基因信号肽序列设计简并引物,从牛蛙皮肤cDNA文库中克隆到两个新的temporins家族抗菌肽,命名为mmporin-La(LLRHVVKILEKUYLarnide)和temporin-Lb (LFRHVVKIFEKYLarnide).合成的temporin-La和temporin-Lb肽具有很强的抗菌活性,尤其是对革兰氏阳性细菌.溶血性测定结果表明,temporin-La浓度高至250mg/L时对兔红细胞仍无溶血活性,而tcmporin-Lb具有较弱的溶血活性(半数致死浓度LC50≈230 μmol/L).通过透射电镜观察temporin-La和temporin-Lb处理过的金黄色葡萄球菌的细胞结构,发现它们都能直接地杀死细菌,但作用机制不一样.
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