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一、同工酶的概念在生物有机体内,能催化同一种反应,但分子结构不同的蛋白质分子叫做同工酶。同工酶在结构上的差异可以在电泳中表现出来,因而容易识别。在五十年代初,Meister(1950)和Neilands(1952)在分离哺乳动物的乳酸脱氢酶时,首先发现同工酶的存在。后来发现同工酶是普遍存在于生物界中,目前在植物中已发现有45
First, the concept of isoenzyme in the biological organisms, can catalyze the same reaction, but the molecular structure of different protein molecules called isozymes. Isoenzyme structural differences can be demonstrated in electrophoresis, and therefore easy to identify. In the early 1950s, Meister (1950) and Neilands (1952) first discovered the presence of isozymes when isolating mammalian lactate dehydrogenases. Later it was found that isozymes are ubiquitous in the biological world, and 45 have been found in plants so far