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对 12个优良玉米自交系的DNA进行了RAPD分析 ,共筛选了 2 5 0个Operon引物 ,其中 12个引物共扩增出 5 9个多态性产物 ,根据这 5 9个多态性RAPD产物 ,对 12个自交系进行了聚类分析 ,把它们分为 3个群 ,结果与根据系谱法的聚类结果一致 .经过优选 ,用OPN - 11扩增出的 11条DNA带 ,建立了这 12个自交系的RAPD -DNA指纹图谱 .在该图谱中每个自交系都具有特异的DNA指纹 ,可以与其它自交系相区别 .然后 ,用 1和 0分别表示各条DNA带的出现和缺失 ,建立了这 12个自交系的计算机化的DNA指纹 ,可用于玉米自交系的真伪及纯度鉴定 .图 4表 1参 6
RAPD analysis of DNA from 12 elite maize inbred lines was carried out. A total of 250 Operon primers were screened out, of which 12 primers amplified a total of 59 polymorphic products. Based on these 59 polymorphic RAPDs The 12 inbred lines were clustered and divided into 3 groups, the results were consistent with the clustering results according to the pedigree method.After optimization, 11 DNA bands amplified by OPN - 11 were established RAPD-DNA fingerprinting of these 12 inbred lines, in which each inbred line has a specific DNA fingerprint that is distinguishable from the other inbred lines, and each DNA is represented by 1 and 0, respectively With the appearance and absence of bands, the computerized DNA fingerprints of these 12 inbred lines were established and could be used for authenticity and purity identification of maize inbred lines.