论文部分内容阅读
用间接免疫荧光染色及微量细胞毒试验证实.人白细胞介素2(Interleukin 2,IL-2)脬育原代培养生长呈融汇状的人脐带静脉内皮细胞24～72小时.可诱发细胞的HLA—DR抗原表达,而内皮细胞抗原和Ⅷ因子相关抗原在这一过程仍呈阳性反应.IL-2影响内皮细胞摄取~8H-TdR和~3H-UR.同时对细胞形态也育影响。T淋巴细胞和经植物凝集素(PHA)激活的T细胞及其条件培养液也影响内皮细胞对~3H-TdR和~3H—UR的摄入.结果提示IL-2不仅可诱发内皮细胞的HLA-DR抗原表达.且影响细胞的代谢.
Indirect immunofluorescence staining and cytotoxicity assays were used to confirm that Interleukin 2 (IL-2) primary cultured human umbilical vein endothelial cells HLA-DR antigen expression, and endothelial cell antigen and factor Ⅷ-related antigen in this process is still positive.IL-2 affect endothelial cells uptake of ~ 8H-TdR and ~ 3H-UR.At the same time, cell morphology also affect. T lymphocytes and PHA-stimulated T cells and their conditioned medium also affected the uptake of ~ 3H-TdR and ~ 3H-UR in endothelial cells.The results suggest that IL-2 can not only induce endothelial cell HLA -DR antigen expression and affect cell metabolism.