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目的对2014-2015年广州市食物中毒检出的44株金黄色葡萄球菌进行肠毒素、耐药研究以及脉冲场凝胶电泳(PFGE)分型,了解其产肠毒素、耐药特性以及同源性。方法依据GB4789.10-2010采用ELISA方法测定金黄色葡萄球菌肠毒素(SEA-SEE),利用VITEK 2 compact对菌株进行药敏试验,并对菌株进行PFGE分型研究。结果 44株金黄色葡萄球菌产肠毒素的有38株(86.4%),其中同时产两种及以上肠毒素型别的有13株(34.2%),产A型肠毒素的菌株最多,有34株(89.5%)。对青霉素(79.5%)、四环素(63.6%)、克林霉素(54.5%)、红霉素(52.3%)耐药率较高。PFGE分型显示菌株可以分为4个聚类、7个PFGE型别。结论食物中毒株产肠毒素能力较强,其对多种抗生素的耐药性提示安全用药的重要性,PFGE分型对广州市金黄色葡萄球菌食物中毒的防控及溯源有重要意义。
Objective To study 44 strains of Staphylococcus aureus isolated from food poisoning in Guangzhou during 2014-2015 for enterotoxigenicity, drug resistance and PFGE genotyping to understand their enterotoxigenicity, drug resistance and their homology Sex. Methods Staphylococcus aureus enterotoxin (SEA-SEE) was determined by ELISA according to GB4789.10-2010. The susceptibility test of the strain was carried out by using VITEK 2 compact. PFGE typing of the strains was also conducted. Results The results showed that 44 strains of Staphylococcus aureus produced 38 strains of enterotoxins (86.4%), among which 13 strains (34.2%) produced more than two kinds of enterotoxigenic strains and the most strains produced type A enterotoxins, 34 Strain (89.5%). The rate of resistance to penicillin (79.5%), tetracycline (63.6%), clindamycin (54.5%) and erythromycin (52.3%) was high. PFGE typing showed that the strains could be divided into four clusters and seven PFGE types. Conclusion The food poisoning strains have strong ability of producing enterotoxins. The drug resistance to multiple antibiotics indicates the importance of safe medication. The classification of PFGE is of great significance in the prevention and control of the food poisoning caused by Staphylococcus aureus in Guangzhou.