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目的分析大肠癌发生过程中p53基因的突变作用,评估外周血p53基因突变分析作为大肠癌高危对象筛查指标的实际应用价值。方法 用聚合酶链反应-单链构象多态(PCR-SSCP)银染技术对50例大肠癌家系成员和20例健康人(对照组)的外周血p53基因第5~8外显子进行检测。结果 大肠癌家系成员和对照组的外周血p53基因突变率为24%和0,差异有显著性(P<0.05)。结论p53基因突变在大肠癌的发生过程中起重要作用,外周血p53基因突变分析可望成为大肠癌高危对象的早期诊断手段。
Objective To analyze the mutation of p53 gene in the process of colorectal carcinogenesis and to evaluate the value of p53 gene mutation in peripheral blood as a screening index for high risk patients with colorectal cancer. Methods The exons 5-8 of p53 gene in peripheral blood of 50 colorectal cancer family members and 20 healthy controls (control group) were detected by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) . Results The mutation rate of p53 gene in peripheral blood of colorectal cancer family member and control group was 24% and 0, the difference was significant (P <0.05). Conclusion The mutation of p53 gene plays an important role in the carcinogenesis of colorectal cancer. The mutation of p53 gene in peripheral blood is expected to be an early diagnostic tool for high-risk patients with colorectal cancer.