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目的:观察长期、大量给予柴胡醇水提取物导致大鼠肝毒性损伤程度和与氧化损伤的相关性。方法:Wistar大鼠按照柴胡总皂苷100、50、25mg/kg连续给药60天,检测血肝功能指标、血和肝组织内MDA含量和SOD活性;检测血中总巯基(-SH)、血和肝组织内谷胱甘肽(GSH)和谷胱甘肽过氧化物酶(GSH-Px)的含量和活性。结果:柴胡醇水提取物可致血ALT、AST增高,血中和肝组织内MDA含量增加,同时SOD活性下降;血中总巯基(-SH)、血和肝组织中谷胱甘肽(GSH)含量下降,谷胱甘肽过氧化物酶(GSH-Px)活性下降。上述变化随剂量增加而加重。结论:柴胡醇水提取物长期给药后可导致大鼠肝毒性损伤,其损伤途径与机体氧化应激后诱导脂质过氧化、组织内活性分子巯基损耗而造成肝组织损伤有关。
OBJECTIVE: To observe the long-term, large-scale administration of aqueous extract of chaiurin to rats resulting in hepatotoxicity and oxidative damage. METHODS: Wistar rats were treated with total saponin 100, 50, and 25 mg/kg for 60 consecutive days. Blood function parameters, MDA content and SOD activity in blood and liver tissues were measured; total sulfhydryl (-SH) levels in blood were measured. The content and activity of glutathione (GSH) and glutathione peroxidase (GSH-Px) in blood and liver tissue. RESULTS: The aqueous extract of Chaihuol induced blood ALT and AST increased, the content of MDA in blood and liver increased, and the activity of SOD decreased. The total sulfhydryl (-SH) in blood, blood and glutathione in liver tissue (GSH) ) The content of glutathione peroxidase (GSH-Px) decreased. The above changes worsened with increasing doses. Conclusion: The long-term administration of Chaihuol water extract can cause hepatotoxicity injury in rats. The damage pathway is related to the lipid peroxidation induced by oxidative stress, and the loss of active thiol in the tissue.