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目的 探讨用一种新建立的外加电场固定液相分子快速斑点免疫分析 (rapidelectricfieldimmobilizingliquidphasemoleculedotblot,REILMD)法检测自身免疫性疾病患者抗内耳抗体的临床意义。方法 收集 11种确诊的自身免疫性疾病患者共 71例 ,突发性聋 2 1例 ,非免疫性疾病患者 48例 (对照组 )。检测内容包括一般免疫指标及自身抗体如抗smoothmuscle (Sm)、抗ribonucleoprotein(RNP)、抗Sj¨ogren’ssyndromeA (SSA)、抗Sj¨ogren’ssyndromeB (SSB)、抗DNAtopoisomeraseI (Scl) 70、抗histidyl tRNAsynthetase (Jo 1)、抗核糖体、抗双链DNA、抗组织和抗内耳抗体。抗内耳抗体检测先用REILMD筛选 ,再用免疫转印方法确定相对分子质量。结果 类风湿性关节炎 (rheumatoidarthritis,RA)和系统性红斑狼疮 (systemiclupuserythematosus ,SLE)主要表现为血沉加快 ,类风湿因子阳性 ,C 反应蛋白、IgG、循环免疫复合物升高 ,少数IgA、IgM及C4 升高。在RA中 2 / 16抗双链DNA和抗线粒体及 4/ 16抗核抗体阳性。在SLE中 11/ 16抗核抗体、7/ 13抗RNP抗体、抗SSA抗体和抗双链DNA抗体 ,3/ 13抗Sm抗体、1/ 13抗ScL70抗体、抗横纹肌和抗胃壁细胞抗体阳性。强直性脊柱炎 (ankylosingspondylitis,AS)均为阴性。经REILMD法筛选共?
Objective To explore the clinical significance of detecting anti-inner ear antibodies in patients with autoimmune diseases by using a newly established electric field fixed liquid molecular rapid spot immuno immunoassay (REILMD). Methods Totally 71 patients with 11 confirmed autoimmune diseases were collected, 21 cases were sudden deafness and 48 cases were non-immune diseases (control group). The tests included general immune indicators and autoantibodies such as anti-smoothmuscle (Sm), anti-ribonucleoprotein (RNP), anti-Sjögrenssyndrome A (SSA), anti-Sjögrenssyndrome B (SSB), anti-DNAtopoisomerase I histidyl tRNAsynthetase (Jo 1), anti-ribosome, anti-dsDNA, anti-tissue and anti-inner ear antibody. Anti-inner ear antibody detection using REILMD screening, and then use the method of immunoblotting to determine the relative molecular mass. Results Rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) mainly manifested as fasting erythrocyte sedimentation rate, rheumatoid factor positive, C reactive protein, IgG, elevated circulating immune complex, few IgA, IgM and C4 rises. 2/16 anti-double stranded DNA and anti-mitochondrial and 4/16 anti-nuclear antibodies were positive in RA. 11/16 anti-nuclear antibody, 7/13 anti-RNP antibody, anti-SSA antibody and anti-double stranded DNA antibody, 3/13 anti-Sm antibody, 1/13 anti-ScL70 antibody, anti-striated muscle and anti-parietal cell antibody were positive in SLE. Ankylosing spondylitis (AS) was negative. A total of screening by REILMD method?