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In the recent decade, plant regeneration fromprotoplast has been obtained through embryo-genic cell suspension cultures of rice. Howev-er, not only the establishment of embryogeniccell suspension cultures of rice was difficult,but also the protoplasts became less and lessregenerable and the genetic change was gradu-
Howev-er, not only the establishment of embryogenic cell suspension cultures of rice was difficult, but also the protoplasts became less and less genetically and the genetic change was gradu-