论文部分内容阅读
“七五”期间,我们采用体内、体外及脾内免疫等多种免疫方法,以大肠癌细胞株及新鲜肠癌组织为免疫原制备了十数株抗大肠癌单克隆抗体,并对抗体进行了较系统的鉴定。血清学诊断已建立反向血凝试验和双夹心酶联免疫吸附试验两种检测方法。用单抗Hb_3共检测了590例1 194份血清样本。结果表明,结直肠癌的阳性率为55.8%和70%,胃癌的阳性率为43.0%和61.5%,胃肠良性疾病的阳性率为9.3%和12.2%,正常献血员阳性率为3.0%和6.7%。研究表明肿瘤相关抗原CA-Hb_3表达的阳性率和平均水平与大肠癌的临床分期相关,Dukes C-D期显著高于Dukes A-B期。跟踪观察161例结直肠癌病人手术前后
During the “Seventh Five-Year Plan” period, we used in vivo, in vitro, and spleen immunization methods to prepare ten strains of colorectal cancer monoclonal antibodies against colorectal cancer cell lines and fresh colorectal cancer tissues as immunogens. A more systematic identification. Serological diagnosis has established two methods of reverse hemagglutination test and double sandwich enzyme-linked immunosorbent assay. A total of 590 1,194 serum samples were tested with monoclonal antibody Hb_3. The results showed that the positive rate of colorectal cancer was 55.8% and 70%, the positive rate of gastric cancer was 43.0% and 61.5%, the positive rate of gastrointestinal benign disease was 9.3% and 12.2%, and the positive rate of normal blood donor was 3.0% and 6.7%. Studies have shown that the positive rate and average expression of tumor-associated antigen CA-Hb 3 is related to the clinical stage of colorectal cancer, and the Dukes C-D stage is significantly higher than the Dukes A-B stage. Follow-up observation of 161 patients with colorectal cancer before and after surgery