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目的 :观察大鼠体表严重烫伤后海马神经元的病理变化 ,探讨CNTF对其保护作用。方法 :SD大鼠侧脑室埋管 ,制成 30 %体表面积Ⅲ度烫伤模型 ;伤后 3d ,测定脑组织含水量 ,海马乳酸脱氢酶 (LDH)和一氧化氮 (NO)的含量 ;做病理切片 ,尼氏染色。结果 :大鼠严重烫伤后 ,脑组织含水量增加 ,海马出现明显的病理变化 ,尼氏小体减少或消失 ,胞体肿胀 ,LDH和NO的含量增加 ;给予CNTF后 ,脑水肿、海马的病理变化有明显改善 ,并能降低LDH和NO的含量。结论 :大鼠严重体表烫伤可造成脑组织损伤 ,CNTF对海马神经元损伤有保护作用 ,这种保护作用可能与其降低NO含量有关。
OBJECTIVE: To observe the pathological changes of hippocampal neurons after severe scalding in rats, and to explore the protective effect of CNTF. Methods: SD rats were implanted into the lateral ventricle to make a third-degree scald model with 30% body surface area. After 3 days, the content of brain water, the contents of LDH and NO in the hippocampus were determined. Pathological sections, Nissl staining. RESULTS: After severe scalds in rats, the water content of brain tissue increased, the pathological changes of hippocampus appeared, the number of Nissl bodies decreased or disappeared, the swelling of body cells and the content of LDH and NO increased. After the administration of CNTF, the pathological changes of cerebral edema and hippocampus Significantly improved, and can reduce the content of LDH and NO. CONCLUSION: Severe scalded rats can cause brain injury. CNTF can protect hippocampus neurons from injury, which may be related to the decrease of NO content.