研究了用高效液相色谱法分析银杏叶提取物中黄酮含量时几个主要环节的操作条件,包括水解液组成,水解温度和时间;论证了在370nm波长下检测时,可用槲皮素一个标准物质方便地求出槲皮素,山萘酚和异鼠李素3种黄酮的含量.水解液是5.5%(W/V)HCI甲醇溶液,在 85～90℃回流1.3 h.采用Bio-Sil ODS 5μm柱,150 ×4.0mm I.D.,甲醇/0.4%(V/V)磷酸水溶液(57:43)流动相,UV-37nm检测.回收率和分析精度令人满意. The main operating conditions of flavonoids in Ginkgo biloba extract were analyzed by high performance liquid chromatography (HPLC), including the composition of the hydrolyzate, the hydrolysis temperature and the time. It was demonstrated that when tested at 370 nm, quercetin could be used as a standard The quercetin, kaempferol and isorhamnetin flavonoids were conveniently obtained from the material.The hydrolyzate was 5.5% (w / v) HCI in methanol and refluxed for 1.3 h at 85-90 ° C. Bio-Sil ODS 5μm column, 150 × 4.0mm ID, methanol / 0.4% (V / V) aqueous phosphoric acid solution (57:43) and UV-37nm detection.The recovery and analytical precision were satisfactory.