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目的:检测胶质瘤组织中转化生长因子β1(TGF-β1)表达,并分析其与CD4+FOXP3+调节性T细胞(Treg)浸润的关系,探讨Treg浸润的临床意义。方法:采用免疫组织化学双标记的方法检测135例胶质瘤组织(WHOⅠ18,WHOⅡ45,WHOⅢ53,WHOⅣ19)、15例正常脑组织中TGF-β1、Treg(CD4+Foxp3+)的表达。结果:胶质瘤组织中TGF-β1高表达77例,低表达58例,高表达率57.03%,正常脑组织中未见表达。胶质瘤组织中Treg细胞平均密度为2.031个/HP,正常脑组织中未见表达。胶质瘤组织中TGF-β1与Treg细胞表达呈正相关(r=0.294,P<0.01)。高级别胶质瘤组织中TGF-β1,Treg的表达明显高于低级别。胶质瘤组织中Treg细胞表达与患者年龄呈正相关而与性别、KPS评分无关。胶质瘤组织中TGF-β1表达高、低水平组患者总生存率差异有统计学意义(P<0.05),TGF-β1高水平组患者的总生存率低于低水平组(25.97%vs36.21%)。多因素Cox回归模型分析显示TGF-β1表达、Treg细胞数量不是影响胶质瘤患者预后的独立危险因素(P>0.05)。结论:胶质瘤组织中Treg浸润可能与TGF-β1表达有关。高级别胶质瘤组织中TGF-β1,Treg的表达显著高于低级别,高表达组的总生存率显著低于低水平组,但TGF-β1,Treg不能做为影响胶质瘤生存时间的独立预后因素。
OBJECTIVE: To detect the expression of transforming growth factor-β1 (TGF-β1) in glioma tissue and analyze its relationship with the infiltration of CD4 + FOXP3 + regulatory T cells (Tregs) and explore the clinical significance of Treg infiltration. Methods: The expressions of TGF-β1, Treg (CD4 + Foxp3 +) in 135 glioma tissues (WHOⅠ18, WHOⅡ45, WHOⅢ53, WHOⅣ19) and 15 normal brain tissues were detected by immunohistochemical double labeling method. Results: There were 77 cases of high expression of TGF-β1 in glioma tissues, 58 cases of low expression, the high expression rate of 57.03%, no expression in normal brain tissue. The average density of Treg cells in glioma tissue was 2.031 / HP, which was not found in normal brain tissue. The expression of TGF-β1 and Treg cells in glioma tissues was positively correlated (r = 0.294, P <0.01). The expression of TGF-β1 and Treg in high-grade glioma tissues was significantly higher than that in low-grade gliomas. The expression of Treg cells in glioma tissues was positively correlated with patient’s age but not with gender and KPS score. The overall survival rate of glioma tissues with high and low TGF-β1 expression was significantly different (P <0.05). The overall survival rate of TGF-β1 high-level group was lower than that of low-level group (25.97% vs36. twenty one%). Multivariate Cox regression analysis showed that the expression of TGF-β1 and the number of Treg cells were not independent risk factors for the prognosis of gliomas (P> 0.05). Conclusion: Treg infiltration in glioma may be related to the expression of TGF-β1. The expression of TGF-β1 and Treg in high-grade glioma tissues was significantly higher than that in low-grade glioma tissues, and the overall survival rate was significantly lower in high-grade glioma tissues than in low-grade glioma tissues Independent prognostic factors.