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目的：本文旨在评价新方法心肌声学造影（ＭＣＥ）在缺血预适应（ＩＰ）研究中的价值及ＩＰ过程中心肌显影动态变化的特征。方法：采用前降支动脉套扎建立ＩＰ开胸犬模型，在１小时的长缺血前预缺血和再灌注各５分钟，反复４次，并于再灌注２小时进行经静脉ＭＣＥ，继而取出心脏用Ｅｖａｎ氏蓝和ＴＴＣ染色，分析两者所代表的坏死面积占缺血危险区面积的百分比（ＡＮ％）关系。同时，分别于基础、缺血前、缺血１小时、再灌注０．５小时和２小时行ＭＣＥ，测量结扎区ＭＣＥ缺损面积进行前后比较，并与缺血再灌注（ＩＲ）对照组比较。结果：（１）ＭＣＥ与组织染色法所测的ＡＮ％相一致；（２）在ＩＰ刺激期，可见最小和最大ＭＣＥ缺损，其最小所占的百分比（ＡＤｍｉｎ％）随ＩＰ刺激而下降；在再灌注期，ＩＰ组ＡＤｍｉｎ％无变化，而ＩＲ组明显升高。结论：ＭＣＥ可作为研究ＩＰ的有效方法，为ＩＰ的在体研究并过渡临床提供了新的方法；ＩＰ可使刺激期心肌无复流范围相对缩小而再灌注期无复流无扩大。
OBJECTIVE: This article aims to evaluate the value of the new method of myocardial contrast echocardiography (MCE) in ischemic preconditioning (IP) and the dynamic changes of myocardial development during IP. Methods: The IP thoracotomy model was established by ligation of the anterior descending artery and pre-ischemic and reperfused for 5 minutes each for 1 hour, repeated 4 times and transvenous MCE 2 hours after reperfusion. The hearts were removed and stained with Evan’s Blue and TTC to analyze the percentage of necrotic area represented by the two as a percentage of area of risk for ischemic attack (AN%). At the same time, MCE was measured before basal ischemia, 1 hour before ischemia, 0.5 hour after reperfusion and 2 hours after reperfusion respectively. The area of MCE defect in the ligation area was measured before and after comparison, and compared with ischemia / reperfusion (IR) control group. Results: (1) The MCE was consistent with the AN% measured by histological staining; (2) The minimum and maximum MCE defects were seen in IP stimulation period, the minimum percentage of which (ADmin%) decreased with IP stimulation; During reperfusion, there was no change in ADmin% in IP group, but significantly increased in IR group. Conclusion: MCE can be used as an effective method to study IP, which provides a new method for in-vivo study and clinical transition of IP. IP can reduce the range of myocardial no-reflow in stimulated myocardium and no-reflow without reperfusion.