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目的探讨地氟醚对新生大鼠学习记忆功能的影响。方法选用健康清洁级雄性SpragueDaw ley(SD)新生6 d大鼠24只,随机分为研究组和对照组,每组各12只,对照组给予体积分数为50%的空气与氧气的混合气体吸入4 h,研究组在对照组的基础上给予质量分数为8.5%的地氟醚麻醉4 h。Morris水迷宫实验检测2周和6周时大鼠的学习记忆功能;6周时应用Western blot和Real-time PCR方法检测大鼠大脑皮质组织的凋亡相关蛋白Caspase-3、Bax和Bcl-2以及w nt通路相关蛋白β-catenin和GSK-3β的蛋白及m RNA水平。结果研究组2周时大鼠的学习记忆功能无显著变化,而6周时受到了抑制,促进细胞凋亡的Caspase-3、Bax表达明显增强,而抑制细胞凋亡的Bcl-2表达下降;wnt通路相关蛋白β-catenin和GSK-3β表达下降。结论地氟醚麻醉可引起新生大鼠远期学习记忆功能损害,导致细胞凋亡,其机制可能与wnt通路相关。
Objective To investigate the effects of desflurane on learning and memory in neonatal rats. Methods Twenty-four newborn Sprague Dawley (SD) rats of healthy and clean grade were randomly divided into study group and control group, with 12 rats in each group. The control group was inhaled with a mixed gas of air and oxygen with a volume fraction of 50% 4 h, the study group on the basis of the control group to give a mass fraction of 8.5% desflurane anesthesia 4 h. Morris water maze test was used to detect the learning and memory function of rats at 2 weeks and 6 weeks. Western blot and Real-time PCR were used to detect the expression of apoptosis-related proteins Caspase-3, Bax and Bcl-2 in cerebral cortex As well as the protein and m RNA levels of wnt pathway related proteins β-catenin and GSK-3β. Results At 2 weeks, the learning and memory abilities of rats in study group were not significantly changed, but were inhibited at 6 weeks. The expression of Caspase-3 and Bax in apoptosis increased significantly, while the expression of Bcl-2 in apoptosis decreased. wnt pathway related protein β-catenin and GSK-3β expression decreased. Conclusion Desflurane anesthesia can cause long-term learning and memory impairment, leading to apoptosis in neonatal rats, which may be related to the wnt pathway.