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Apoptosis in hippocampal neurons is an important contributor to depression.[Ca2+]i of hippocampal neurons may raise rapidly with increased corticosterone concentrations,leading to apoptosis.The anti-depressant effects of Xiaoyaosan (Free and Easy Wanderer’s Powder) may inhibit apoptosis by down regulating the [Ca2+]i overload.PC12 cells were containing with corticosterone to simulate neuronal injury,dosed with Xiaoyaosan-containing serum,and the following parameters were measured: apoptosis,[Ca2+]i overload,and changes in mitochondrial membrane potential (?ψm) and bioenergetics.High-dose Xiaoyaosan-containing serum (the dose of intragastric administration was 2.633 g herb/mL) not only inhibited morphological injury and cell apoptosis,but also suppressed [Ca2+]i overload induced by corticosterone.Xiaoyaosan also blocked the decrease in mitochondrial membrane potential and adenosine triphosphate levels induced by corticosterone.Xiaoyaosan dose-dependently suppresses corticosterone-induced apoptosis,potentially via inhibiting [Ca2+]i overload,and prevents impaired mitochondrial bioenergetics by maintaining mitochondrial membrane potential and adenosine triphosphate production,providing a putative mechanism for its antidepressant effects.
[Ca2 +] i of hippocampal neurons may raise rapidly with increased corticosterone concentrations, leading to apoptosis. The anti-depressant effects of Xiaoyaosan (Free and Easy Wanderer’s Powder) may inhibit apoptosis by down regulating the [Ca2 +] i overload, and changes in mitochondrial membrane potential (? ψm) and bioenergetics. High-dose Xiaoyaosan-containing serum (the dose of intragastric administration was 2.633 g herb / mL) not onlymorph morphological injury and cell apoptosis, but also suppressed [Ca2 +] i overload induced by corticosterone. Xiaoyaosan also blocked the decrease in mitochondrial membrane potential and adenosine triphosphate levels induced by corticosterone. Xiaoyaosan dose-dependently suppresses corticosterone-ind uced apoptosis, potentially via inhibiting [Ca2 +] i overload, and prevents impaired mitochondrial bioenergetics by maintaining mitochondrial membrane potential and adenosine triphosphate production, providing a putative mechanism for its antidepressant effects.