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目的:探讨survivin和bax基因在白毛藤总苷诱导人乳腺癌系MCF-7细胞凋亡中的作用。方法:不同浓度白毛藤总苷作用于MCF-7细胞48 h后,荧光显微镜和流式细胞术法检测MCF-7细胞凋亡情况,Western印迹法及RT-PCR法检测survivin和bax基因表达量的变化。结果:白毛藤总苷能诱导MCF-7细胞凋亡,并且上调bax和降低survivin表达。结论:白毛藤总苷能促进MCF-7细胞凋亡,其机制可能与激活bax和抑制survivin表达有关。
Objective: To investigate the role of survivin and bax gene in the apoptosis of human breast cancer MCF-7 cells induced by total glycosides of Ctenopha canerea. Methods: The apoptosis of MCF-7 cells was detected by fluorescence microscope and flow cytometry after treated with different concentrations of total glycoside of MCT-MCF-7 cells for 48 h. The expressions of survivin and bax gene were detected by Western blotting and RT-PCR The amount of change. Results: Total glycosides of Cortex Fragrans could induce the apoptosis of MCF-7 cells and up-regulate the expression of bax and decrease the expression of survivin. Conclusion: The total glycoside of Cynanchum paniculatum can promote the apoptosis of MCF-7 cells, which may be related to the activation of bax and the inhibition of survivin expression.