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目的利用自旋标记—顺磁共振技术研究严重烧伤大鼠早期心肌细胞膜的变化。方法用5-氮氧自由基硬脂酸(5-doxylstearic acid,5NS)和16-氮氧自由基硬酯酸((16-doxylstearic acid,16NS)分别掺入到不同深度的膜类脂区,观察严重烧伤对大鼠心肌细胞不同深度的膜类脂区流动性的影响,同时用3-甲基马来酰亚胺氮氧自由基(3-maleidoproxy1,3MP)标记膜蛋白巯基,观察膜蛋白旋转相关时间(τ_c)及强固定化与弱固定化组分谱线高度比值(h_s/h_w)的变化。结果严重烧伤大鼠早期心肌细胞膜脂浅层的序参数(S)值无明显变化,而膜脂深层 S 值增大,膜蛋白 h_s/h_w 显著增大,膜蛋白旋转相关时间τ。也显著增大。结论严重烧伤后早期即出现心肌细胞膜的损伤,表现为膜脂质深层流动性下降,膜蛋白构象改变,膜蛋白运动受限。这些变化可以直接影响细胞膜蛋白的功能,从而导致心肌细胞功能的变化。
Objective To study the changes of early myocardial cell membrane in severely burned rats by spin-labeling-paramagnetic resonance technique. Methods 5-doxylstearic acid (5NS) and 16-doxylstearic acid (16NS) were respectively incorporated into membrane lipid regions with different depths. To observe the effect of severe burn on the fluidity of membrane lipids at different depths in rat cardiomyocytes and the membrane protein thiols were labeled by 3-maleidoproxy 1,3MP (Τ_c), and the ratio of h_s / h_w to the line height ratio (h_s / h_w) of the strongly immobilized and weakly immobilized components.Results There was no significant change in order parameter (S) While the S value of membrane lipid increased, the membrane protein h_s / h_w increased significantly, and the relative time τ of membrane protein rotation also increased significantly.Conclusion Myocardial cell membrane damage occurs early after severe burn, which is manifested as membrane lipid fluidity Decreased, conformational change of membrane protein, restricted movement of membrane protein.These changes can directly affect the function of cell membrane proteins, leading to changes in myocardial cell function.