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目的探讨3种免疫抑制药(雷帕霉素、环孢霉素A和紫杉醇)在骨髓前体细胞分化过程中对分化的巨噬细胞表型和功能的影响。方法取60只C57BL/6小鼠颈椎脱臼致死,无菌操作制备骨髓前体细胞,在细胞培养体系中分别加入雷帕霉素(100μmol/L)、环孢霉素A(1mg/L)和紫杉醇(20μg/L)和巨噬细胞集落刺激因子,通过混合淋巴细胞反应和流式细胞仪分析分化的巨噬细胞表型和功能。结果雷帕霉素处理组分化的巨噬细胞表面分子CD80和CD54表达明显降低;环孢霉素A处理组巨噬细胞表面分子CD80、CD40和I-Ad表达显著降低,而CD86表达明显增加;紫杉醇处理组巨噬细胞表面分子CD80、CD86和CD14表达显著增加,而I-Ad表达明显降低。雷帕霉素、环孢霉素A处理组分化的巨噬细胞吞噬鸡红细胞能力和抗原递呈能力明显降低,而紫杉醇处理组的巨噬细胞吞噬功能和分泌IL-12能力明显强于对照组,但免疫原性降低。结论 3种免疫抑制药可能影响体外分化的巨噬细胞表型和功能。
Objective To investigate the effects of three immunosuppressive drugs (rapamycin, cyclosporin A and paclitaxel) on the phenotype and function of differentiated macrophages in differentiation of bone marrow progenitor cells. Methods Sixty C57BL / 6 mice were killed by cervical dislocation and aseptically treated with 100 ug / L rapamycin (1 mg / L) and Paclitaxel (20 μg / L) and macrophage colony-stimulating factor were used to analyze the phenotype and function of differentiated macrophages by mixed lymphocyte reaction and flow cytometry. Results The expression of CD80 and CD54 on the surface of macrophages were significantly decreased after rapamycin treatment. The expression of CD80, CD40 and I-Ad on macrophages was significantly decreased and the expression of CD86 on macrophages was significantly increased after cyclosporin A treatment. Paclitaxel treatment group macrophages surface molecules CD80, CD86 and CD14 expression was significantly increased, while I-Ad expression was significantly reduced. Rapamycin and cyclosporine A treatment of macrophages phagocytosis of chicken erythrocytes and antigen presentation ability was significantly reduced, while paclitaxel-treated macrophages phagocytosis and secretion of IL-12 ability was significantly stronger than the control group , But reduced immunogenicity. Conclusion The three immunosuppressive drugs may affect the phenotype and function of macrophages differentiated in vitro.