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以甘油(Gly)为抗冻剂、0.5 mL麦细管为冻存管,两步降温法超低温冷冻保存大黄鱼精子,用单细胞凝胶电泳(SCGE)法检测冻精核的DNA损伤。结果表明:以Cortland液为稀释溶液,5%~20%Gly为抗冻剂,超低温冷冻保存大黄鱼精子的效果较佳,冻精活力与鲜精活力无显著差异;Gly浓度为10%,冻精的激活率和运动时间分别达89.93%和8.91min;25%、30%Gly组冻精活力显著下降;SCGE检测显示,Gly浓度5%~20%时,冻精核DNA损伤与鲜精无显著差异,Gly浓度25%及30%时,冻精核DNA损伤与鲜精差异显著;鲜精与冻精的DNA损伤主要表现为轻度和中度损伤,重度损伤比率较低,完全损伤只存在于25%Gly及30%Gly组的冻精中,且比例低。
Cryoprecipitate DNA damage was detected by single cell gel electrophoresis (SCGE) with glycerol (Gly) as cryoprotectant and 0.5 mL as cold storage tube. Cryopreservation of large yellow croaker by two-step cooling method was carried out. The results showed that 5% -20% Gly was used as antifreeze solution with Cortland solution as the dilute solution, cryopreservation of sperm of big yellow croaker was better, and no significant difference was found between vitrification activity and fresh vitality. Gly concentration was 10%, frozen The activation rate of sperm and exercise time were 89.93% and 8.91min, respectively. The sperm motility of 25% and 30% Gly groups decreased significantly. The SCGE assay showed that the DNA damage of sperm nucleus and spermine Significant difference, Gly concentration of 25% and 30%, frozen sperm DNA damage and fresh fine difference significantly; fresh and frozen sperm DNA damage mainly for mild and moderate injury, low rate of severe injury, complete injury Presence in 25% Gly and 30% Gly groups of frozen semen, and the proportion of low.