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目的 应用同位素相对标记与绝对定量技术(iTRAQ)分析β-地中海贫血患者血浆蛋白,筛选出差异蛋白及其功能分析.方法 通过强阳离子交换色谱分析法的8种不同同位素标记的iTRAQ和液相色谱法以及串联质谱分析了地贫组和健康照者血浆蛋白.通过Mascot软件分析和GO分析表达差异的蛋白.结果 与对照组相比,实验组中共鉴定出有定量信息的蛋白质58个,包括49个上调蛋白和9个下调蛋白.生物信息学分析显示差异表达的蛋白主要参与凝血、凝固止血、伤口愈合、应对受伤、体液调节的水平、急性炎症反应、囊泡、介导转运、血小板激活、血小板脱粒等过程,这些表达差异的蛋白中选择5个显著表达差异作为候选蛋白,分别是CRP、SAA1、HBA、FBLN1、HRG.结论 本研究发现这5种蛋白在β-地中海贫血发病过程中发挥重要作用,可能作为该病潜在的临床筛查生物标志物.“,”Objective:Using isobaric tags for relative and absolute quantification (iTRAQ) technology to analyze the proteins in serum of β-thalassaemias and find the differentially expressed proteome.Methods:Analyzing the total proteins in serum samples from patients with β-thalassaemias and samples of healthy controls by using eight-plex iTRAQ added with strong cation exchange choematography and liquid chromatography coupled with tandem mass spectrometry.The differentially expressed proteins were identified by Mascot and GO.Results:A total of 58 differentially expressed proteins were identified,including 49 proteins were up-regulation and 9 proteins were down-regulation.Bioinformatics analysis revealed that differential expression proteins were mainly related to the blood coagulation Coagulation,hemostasis,wound healing,response to wounding,regulation of body fluid levels,acute inflammatory response,vesicle-mediated transport,platelet activation,and platelet degranulation process.Among of them,there were 5 proteins significant differentially expressed,including CRP,SAA1,HBA,FBLN1,and HRG.Conclusion:This study found that 5 kinds of protein in children with mycoplasma pneumonia play an important role in the process,promisingly as the disease clinical screening of potential biomarkers.