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采用逆转录聚合酶链式反应(RT-PCR),以总RNA作为模板两步法扩增hsp70。设置阳性(热应激)和阴性(蒸馏水培养)对照组,以actin为内参,研究硝酸铈[Ce(NO3)3]胁迫(浓度为1、4、16、64、256mg/L)下玉米根尖细胞hsp70 mRNA表达量的变化。结果表明,Ce(NO3)3各处理组的hsp70 mRNA的表达量高于阴性对照组,1～64mg/L处理组hsp70 mRNA的表达量低于阳性对照组,256mg/L处理组hsp70 mRNA的表达量高于阳性对照组,说明Ce(NO3)3可诱导玉米根尖细胞中hsp70 mRNA的表达。
Reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify hsp70 using total RNA as a template. The positive (heat stress) and negative (distilled water) control groups were set up to study the effects of cerium nitrate [Ce (NO3) 3] stress (1, 4, 16, 64 and 256 mg / L) Changes of hsp70 mRNA expression in spiked cells. The results showed that the expression of hsp70 mRNA in Ce (NO3) 3 treatment group was higher than that in negative control group. The expression of hsp70 mRNA in 1 ~ 64 mg / L treatment group was lower than that in positive control group. The expression of hsp70 mRNA in 256 mg / L treatment group Higher than the positive control group, indicating that Ce (NO3) 3 can induce the expression of hsp70 mRNA in the root tip cells of corn.