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热激蛋白90(Heat shock protein 90,Hsp90)是植物应对不良环境胁迫产生的一类特定的抗逆蛋白。文章以番茄(Solanum lycopersicum L.)基因组数据为平台,借助生物信息学方法对Hsp90基因家族进行鉴定与分析。结果表明,番茄至少含有7个Hsp90基因,不均匀分布在6条染色体上,氨基酸序列长度为267~794aa,内含子数目为2~19;共线性分析发现两对基因(Hsp90-1和Hsp90-3,Hsp90-5和Hsp90-7)以片段重复形式存在。MEME(Multiple Em for Motif Elicitation)分析显示,番茄Hsp90基因编码的氨基酸序列具有多个保守基序;聚类分析揭示番茄、水稻(Oryza sativa L.)和拟南芥(Arabidopsis thaliana L.)Hsp90基因可以分为5组,存在3对直系同源基因和4对旁系同源基因;基于RNA-seq数据库表达分析发现,3个基因(Hsp90-5、Hsp90-6和Hsp90-7)在营养器官和生殖器官中表达量较高,4个基因(Hsp90-1、Hsp90-2、Hsp90-3和Hsp90-4)除在番茄转色后10 d的果实中表达量较高外,其余组织中表达量均较低;对Hsp90基因启动子序列进行分析,发现了多个参与植物对逆境胁迫的顺式作用元件,如HSE、CCAAT-box。此外,qRT-PCR检测结果表明,在叶片热胁迫条件下,番茄Hsp90基因的表达量均存在增强趋势,表明这些基因参与了番茄叶片应对高温胁迫的反应。研究结果为鉴定番茄Hsp90基因的功能和进化起源奠定了基础。
Heat shock protein 90 (Hsp90) is a kind of specific anti-aging protein produced by plants in response to adverse environmental stress. Based on the genomic data of Solanum lycopersicum L., the Hsp90 gene family was identified and analyzed by bioinformatics methods. The results showed that tomato contains at least 7 Hsp90 genes, which are heterogeneously distributed on 6 chromosomes. The length of amino acid sequence is 267 ~ 794aa, and the number of intron is 2 ~ 19. Two genes (Hsp90-1 and Hsp90 -3, Hsp90-5 and Hsp90-7) exist in a repeating pattern of fragments. The analysis of MEM (Multiple Em for Motif Elicitation) showed that the amino acid sequence encoded by tomato Hsp90 gene has multiple conserved motifs. Clustering analysis revealed that the Hsp90 gene of tomato, rice (Oryza sativa L.) and Arabidopsis thaliana L. There are 3 pairs of orthologous genes and 4 pairs of paralogous genes. Based on the RNA-seq database, three genes (Hsp90-5, Hsp90-6 and Hsp90-7) were found in vegetative organs (Hsp90-1, Hsp90-2, Hsp90-3 and Hsp90-4) were expressed in the fruits of tomato at 10 d after transfection, and the expression levels of the other four genes (Hsp90-1, Hsp90-2, Hsp90-3 and Hsp90-4) The results showed that there were many cis-acting elements such as HSE and CCAAT-box, which were involved in plant stress response to stress. In addition, the results of qRT-PCR indicated that the expression of Hsp90 in tomato leaves tended to increase under the conditions of leaf heat stress, indicating that these genes are involved in the response of tomato leaves to high temperature stress. The results laid the foundation for identifying the function and evolutionary origin of tomato Hsp90 gene.