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目的研究黄芪多糖联合丹参酮对心力衰竭(CHF)模型大鼠心肌核因子-κB(NF-κB)通路过度激活的影响。方法以大鼠左冠状动脉结扎术造成心肌梗死后心衰模型。超声心动图观察心功能变化情况,将成模后大鼠分为6组:模型组、黄芪多糖组、丹参酮组、黄芪多糖加丹参酮组、洛伐他汀组、假手术组。光镜下观察心肌病理形态学变化,免疫组化法检查心肌NF-κB的激活,Western blot法检测心肌NF-κB、抑制蛋白IKB及IKB激酶(IKK)蛋白的表达。结果心肌NF-κB被激活,NF-κB的表达明显升高,而IKB及IKK的表达明显降低,应用药物干预后,NF-κB的表达降低,而IKB及IKK的表达升高,差异有显著性(P<0.05)。结论应用益气活血中药对CHF大鼠心肌的心功能损害有一定防治作用,机制可能与抑制NF-κB转导通路过度激活有关。
Objective To investigate the effect of astragalus polysaccharide combined with tanshinone on myocardial NF-κB pathway over-activation in heart failure (CHF) model rats. Methods The left coronary artery ligation in rats resulted in a model of heart failure after myocardial infarction. The changes of cardiac function were observed by echocardiography. The rats were divided into 6 groups: model group, Astragalus polysaccharide group, tanshinone group, Astragalus polysaccharide plus tanshinone group, lovastatin group and sham operation group. The pathological changes of myocardium were observed under light microscope. The activation of NF-κB was detected by immunohistochemistry. The expressions of NF-κB, IKB and IKK were detected by Western blot. Results Myocardial NF-κB activation, NF-κB expression was significantly increased, and IKB and IKK expression was significantly reduced, the application of drug intervention, NF-κB expression decreased, and IKB and IKK expression was significantly increased, the difference was significant (P <0.05). Conclusion The application of Yiqi Huoxue Chinese medicine has some preventive and therapeutic effects on cardiac dysfunction induced by CHF in rats. The mechanism may be related to the over-activation of NF-κB transduction pathway.