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目的 研究中药复方“冬夏丸”体外对Epstein Barr病毒 (EBV)抗原表达的影响。方法应用细胞培养和间接免疫酶技术。结果 2 0～ 80 μg/ml中药复方“冬夏丸”与靶细胞共培养能显著的抑制巴豆油和正丁酸联合诱发Raji细胞的EBV早期抗原 (EBV EA)和B95 8细胞的EBV壳抗原 (EBV VCA)表达及B95 8细胞的EBV VCA自然表达 ,抑制率分别是 19 31%～ 49 84% ,34 6 3%～ 45 6 1%和2 1 6 7%～ 47 78%。用该药预处理Raji和B95 8细胞亦能显著地抑制巴豆油和正丁酸联合诱发EBV EA和VCA的表达。结论 中药复方“冬夏丸”具有明显抑制EBV抗原表达的作用 ,有可能用于EBV相关疾病的防治。
Objective To study the effect of Chinese compound “Wu Xia Pill” on the expression of Epstein Barr virus (EBV) antigen in vitro. Methods Cell culture and indirect immunoenzymatic techniques were used. RESULTS Co-culture of 20-80 μg/ml compound “Feng Xia Pill” with target cells significantly inhibited the EBV early antigen (EBV EA) induced by Croton oil and n-butyric acid in Raji cells and the EBV antigen of B95 8 cells (EBV VCA expression and natural expression of EBV VCA in B95 8 cells were inhibited by 19 31% to 49 84%, 3 64 3% to 45 61%, and 2 1 6 7% to 47 78%, respectively. Pretreatment of Raji and B95 8 cells with this drug also significantly inhibited the expression of EBV EA and VCA induced by croton oil and n-butyric acid. Conclusion The Chinese compound “Wu Xia Pill” has obvious effect on inhibiting the expression of EBV antigen and may be used for the prevention and treatment of EBV-related diseases.