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1 实验材料1.1 拒霜花提取液 将采集的拒霜花叶、花阴干,除去杂物,切段,水煎煮3次(时间分别为1h、1h、30min),合并煎煮液,过滤,滤液乙醇沉淀,过滤,滤液回收乙醇。水溶液浓缩为1:1浓度,分装、灭菌备用。1.2 庆大霉素标准品(批号3268811,642u/mg)。由中国药品生物制品检定所提供。1.3 试验菌株 G~+细球菌属金黄色葡萄球菌[CMCC(B)26003],G~-假单孢菌属绿脓杆菌[CMCC(B)10104],G~-芽胞杆菌属短小芽胞杆菌[CMCC(B)632002],G~-肠杆菌科大肠杆菌[CMCC(B)44102],假单丝酵母菌属真菌白色念珠菌。均由中国药品生物制品检定所提供。
1 Experimental material 1.1 The frost-removing flower extract will collect the frost-repellent mosaics and flowers and dry them, remove the impurities, cut the sections, and decoct the water 3 times (times 1h, 1h, 30min respectively), and combine the decoction liquids and filter them. The filtrate was ethanol precipitated, filtered and the filtrate recovered ethanol. Aqueous solution is concentrated to a 1:1 concentration, and is stored and sterilized for use. 1.2 Gentamicin standard product (lot number 3268811, 642u/mg). Provided by China National Institute for the Control of Pharmaceutical and Biological Products. 1.3 Test strain G~+ Micrococcus Staphylococcus aureus [CMCC(B)26003], G~-Pseudomonas Pseudomonas aeruginosa [CMCC(B)10104], G~-Bacillus pumilus [ CMCC (B) 632002], G~- Enterobacteriaceae Escherichia coli [CMCC (B) 44102], Pseudomonospora fungus Candida albicans. All provided by China National Institute for the Control of Pharmaceutical and Biological Products.