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目的调查广泛耐药鲍曼不动杆菌(Extensively drug Resistant Acinetobacter Baumannii,XDR-ABA)临床分离菌株中氨基糖苷类修饰酶基因、16SrRNA甲基化酶基因和外排泵基因的存在情况。方法收集2011年1—12月临床标本中分离的XDR-ABA菌20株,采用微量肉汤稀释法进行抗菌药物敏感性试验,聚合酶链反应(PCR)方法分析9种氨基糖苷类修饰酶基因、6种16SrRNA甲基化酶基因和外排泵基因adeB。结果 20株XDR-ABA菌均检出aac(6’)-Ⅰb、ant(2“)-Ⅰ、ant(3”)-Ⅰ、aphA1和adeB外排泵基因,其余5种氨基糖苷类修饰酶基因和6种16SrRNA甲基化酶基因和均未检出。结论该组20株XDR-ABA菌耐多种氨基糖苷类药物与细菌产aac(6’)-Ⅰb、ant(2“)-Ⅰ、ant(3”)-Ⅰ、aphA1 4种氨基糖苷类修饰酶和存在adeABC外排泵系统相关。
Objective To investigate the presence of aminoglycoside modifying enzyme gene, 16SrRNA methylase gene and efflux pump gene in clinical isolates of Extensively drug Resistant Acinetobacter Baumannii (XDR-ABA). Methods Twenty strains of XDR-ABA isolated from clinical specimens from January to December in 2011 were collected. Antibacterial susceptibility test was carried out by micro broth dilution method. Nine kinds of aminoglycoside modifying enzyme genes were analyzed by polymerase chain reaction (PCR) , Six kinds of 16SrRNA methylase genes and efflux pump gene adeB. Results The genes of efflux pump of aac (6 ’) - Ⅰb, ant (2’ ’) - Ⅰ, ant (3’ “) - Ⅰ, aphA1 and adeB were detected in all 20 strains of XDR - ABA bacteria and the other 5 aminoglycosides Both the modified enzyme gene and the six 16SrRNA methylase genes were not detected. Conclusion A total of 20 strains of XDR-ABA were resistant to aminoglycosides and 4 kinds of aminoglycosides including aac (6 ’) - Ⅰb, ant (2 ”) - Ⅰ, ant Class-modified enzymes are associated with the presence of adeABC efflux pump system.