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目的 以人卵巢转移瘤及癌旁正常组织为研究对象 ,探求DNA切除修复基因ERCC2 表达与人卵巢转移瘤发生、预后的关系。方法 分别取 1 9例手术卵巢转移瘤及癌旁正常组织各 1 0 0mg ,提取组织中的总RNA ,采用RT PCR的方法分析细胞中ERCC2 表达水平。结果 5例标本中 ,ERCC2 在卵巢转移瘤表达显著低于癌旁正常组织 ,其它 1 4例标本无明显差异。结论 ERCC2 在部分卵巢转移瘤病人中低表达可能是这些卵巢转移瘤病人恶变形成的基础 ,其预后将会与其他病人不同
Objective To investigate the relationship between the expression of ERCC2 gene and the occurrence and prognosis of human ovarian metastases in human ovarian metastases and adjacent normal tissues. Methods Totally 109 cases of ovarian metastases and adjacent normal tissues were collected respectively. The total RNA was extracted and the expression of ERCC2 was analyzed by RT-PCR. Results In 5 specimens, the expression of ERCC2 in ovarian metastases was significantly lower than that in adjacent normal tissues, while the other 14 specimens showed no significant difference. Conclusion The low expression of ERCC2 in some patients with ovarian metastases may be the basis of malignant transformation in these patients with ovarian metastases, the prognosis will be different from other patients