以大孔树脂NKA吸附固定化脂肪酶为催化剂,以月桂酸和植物甾醇为反应物,在无溶剂体系中合成植物甾醇酯;以NKA为载体共吸附固定葡聚糖与酶,考察了反应温度、底物摩尔比、糖种类及葡聚糖添加量和反应时间对酶催化性能的影响.结果表明,在酸/醇摩尔比6:1、葡聚糖与酶共同吸附固定时分子量为10 kDa的葡聚糖添加量为酶量7.5%的条件下,60℃下反应12 h,酯化率高达96.2%,酯化反应时间比简单吸附固定化酶缩短4 h. Macroporous resin NKA adsorption immobilized lipase as catalyst, lauric acid and phytosterol as reactants in the solvent-free system of phytosterol ester synthesis; NKA as a carrier co-immobilization of glucan and enzyme, investigated the reaction temperature , Substrate molar ratio, sugar type and dextran addition amount and reaction time on the catalytic performance of the enzyme were studied.The results showed that the molecular weight of dextran was 10 kDa Of dextran was 7.5%, the esterification rate was 96.2% at 60 ℃ for 12 h, and the esterification reaction time was shortened by 4 h compared with the simple immobilized enzyme.