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目的观察岩黄连生物总碱对肝纤维化大鼠TGF-β1及MMP-9表达的影响,并探讨其抗纤维化的可能机制。方法Wistar大鼠80只随机分为6组,除正常组外其他各组首次皮下注射50%CCl45 ml.kg-1,以后每3天注射1次30%CCl43 ml.kg-1。正常组给予等体积花生油,持续12周,给药自造模之日开始。结果CCl4所致慢性肝纤维化大鼠有明显的肝损伤及慢性肝纤维化的表现。与模型组相比,岩黄连生物总碱三个剂量组能不同程度改善肝功能指标;肝组织中Hyp含量较模型组明显降低;病理组织学检查表明其肝脏病变程度较模型组明显减轻;肝组织中TGF-β1,MMP-9的表达较模型组明显下降。结论岩黄连生物总碱对慢性肝纤维化大鼠具有肝保护作用及抗肝纤维化作用,可能通过抑制TGF-β1和MMP-9表达而促进肝纤维化的逆转。
Objective To observe the effect of total alkaloids from Rhizoma coptidis on the expression of TGF-β1 and MMP-9 in rats with hepatic fibrosis and to explore the possible mechanism of anti-fibrosis. Methods 80 Wistar rats were randomly divided into 6 groups. In addition to the normal group, 50% CCl45 ml.kg-1 was subcutaneously injected for the first time, and 30% CCl43 ml.kg-1 was injected once every 3 days. The normal group was given an equal volume of peanut oil for 12 weeks, starting on the day of administration. Results Chronic liver fibrosis in rats induced by CCl4 showed obvious liver injury and chronic liver fibrosis. Compared with the model group, the three doses of Alkaloids from Rhizoma Coptidis could improve the liver function index in different degrees; the Hyp content in the liver tissue was significantly lower than that in the model group; histopathological examination showed that the liver lesions were significantly reduced compared with the model group; The expression of TGF-β1 and MMP-9 in the tissue was significantly lower than that in the model group. Conclusion The total alkaloids of Rhizoma Sparganii have liver protective effect and anti-fibrosis effect on rats with chronic hepatic fibrosis, which may promote the reversal of hepatic fibrosis by inhibiting the expression of TGF-β1 and MMP-9.