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为揭示光温敏雄性不育小麦恢复系资源的遗传基础,选用49个Genomic-SSR和40个EST-SSR标记位点对100份冬性光温敏雄性不育小麦恢复系进行遗传多样性和群体结构分析.结果表明:89个位点共扩增出531个等位变异,平均每个位点5.96个;平均基因多样性和多态性信息量(PIC)分别为0.63和0.57,说明本研究所选用的恢复系的遗传多样性比较丰富;利用NJ法聚类和Structure群体结构分析均将100份恢复系划分为6大类群,且聚类结果基本吻合,同时揭示出北方冬麦区和黄淮海冬麦区恢复系间存在较广泛的基因交流;群体结构分析阐明了各恢复系的遗传组成,推测58%的恢复系血缘相对比较单一,42%的恢复系拥有混合来源.研究结果为新恢复系的选育和现有恢复系的利用提供了理论依据,并为重要农艺性状数量性状位点的关联分析奠定了基础.
In order to reveal the genetic basis of the restorer lines of photosensitive male sterile wheat, 49 genomic-SSRs and 40 EST-SSR markers were used to analyze the genetic diversity of 100 restorer lines of winter photoperiod- The results showed that 531 alleles were amplified in 89 loci, with an average of 5.96 loci per locus. The average PIC and PIC were 0.63 and 0.57 respectively, The restorer lines selected by the research had rich genetic diversity. Using NJ method and Structure analysis, 100 restorer lines were divided into 6 groups, and the clustering results were basically consistent. Meanwhile, Huanghuaihai and Dongmai restorer lines had a wide range of gene exchange, and the population structure analysis clarified the genetic composition of each restorer line, suggesting that 58% of the restorer lineages were relatively single and 42% of the restorer lines had mixed sources.The results were The establishment of new restorer lines and the utilization of existing restorer lines provided theoretical basis and laid the foundation for the correlation analysis of quantitative trait loci of important agronomic traits.