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研究乳腺癌肿瘤组织中ＮＭ２３－Ｈ１基因蛋白表达，探讨该基因的蛋白表达水平与肿瘤腋下淋巴结转移、肿瘤直径、血管密度以及雌激素受体状况等多项临床病理指标之间的关系。方法应用ＮＭ２３－Ｈ１单克隆抗体以及第８因子相关抗原多克隆抗体免疫组织化学酶标ＡＢＣ法对６０例乳腺癌组织、２０例乳腺纤维腺瘤及２０例癌旁正常乳腺组织中的肿瘤转移抑制基因蛋白产物以及肿瘤新生血管进行了标记。结果ＮＭ２３－Ｈ１蛋白在正常乳腺组织以及乳腺纤维腺瘤的导管上皮中均有高水平表达，乳腺癌肿瘤组织中ＮＭ２３－Ｈ１基因存在较高频率的表达（８８．３％），但表达水平存在差异：高水平表达百分率为４８．３％（２９／６０），低水平表达百分率为５１．６％（３１／６０），腋下淋巴结转移阳性组病例高水平表达率为１２．９％（４／３１），腋下淋巴结转移阴性组病例则为８６．２％（２５／２９）。ＮＭ２３－Ｈ１基因表达水平，腋下淋巴结转移阴性组显著高于腋下淋巴结转移阳性组（Ｐ＜０．０１），并且该基因在肿瘤直径大于２ｃｍ，新生血管密度高于平均值组常呈低水平表达，而不同年龄，雌激素受体状况以及不同病理类型之间该基因的表达水平无差异（Ｐ＞０．０５）。结论ＮＭ２３－Ｈ１基因在控制乳腺癌?
To investigate the expression of NM23-H1 protein in breast cancer tissues and to explore the relationship between the protein expression level and many clinicopathological parameters such as axillary lymph node metastasis, tumor diameter, vascular density and estrogen receptor status. Methods The tumor metastasis in 60 cases of breast cancer, 20 cases of breast fibroadenoma and 20 cases of adjacent normal breast tissues were detected by using NM23-H1 monoclonal antibody and polyclonal antibody of 8-factor related antigen by immunohistochemical ABC method Gene protein products as well as tumor neovascularization were labeled. Results NM23-H1 protein was highly expressed in normal breast tissue and ductal epithelium of breast fibroadenoma. The expression of NM23-H1 gene in breast cancer tissue was higher than that in normal breast tissue (88.3%), but the expression level existed The difference was high (48.3%, 29/60) and low (51.6%, 31/60), respectively. The positive rate of axillary lymph node metastasis was 12.9% (4 / 31), and 86.2% (25/29) cases of negative axillary lymph node metastasis. The expression level of NM23-H1 gene in axillary lymph node metastasis group was significantly higher than that in axillary lymph node metastasis group (P <0.01). The expression of NM23-H1 gene was significantly lower than that in the mean group when the tumor diameter was larger than 2 cm (P> 0.05). However, there was no difference in the expression of estrogen receptor between different age groups and different pathological types (P> 0.05). Conclusion NM23-H1 gene in the control of breast cancer?